Mechanisms and energetics of free radical initiated disulfide bond cleavage in model peptides and insulin by mass spectrometry† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc01305d Click here for additional data file.

Rhodium(i)-catalyzed stereoselective [4+2] cycloaddition of oxetanols with alkynes through C(sp3)–C(sp3) bond cleavage† †Electronic supplementary information (ESI) available. CCDC 1512439 and 1512440. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c6sc05246k Click here for additional data file. Click here for additional data file.

Mechanisms and energetics of free radical initiated disulfide bond cleavage in model peptides and insulin by mass spectrometry.

We investigate the mechanism of disulfide bond cleavage in gaseous peptide and protein ions initiated by a covalently-attached regiospecific acetyl radical using mass spectrometry (MS). Highly selective S-S bond cleavages with some minor C-S bond cleavages are observed by a single step of collisional activation. We show that even multiple disulfide bonds in intact bovine insulin are fragmented ...

Atmospheric pressure neutral reionization mass spectrometry for structural analysis† †Electronic supplementary information (ESI) available: Details for experimental procedures and additional supporting data. See DOI: 10.1039/c7sc01999h Click here for additional data file.

Ion dissociation is the usual basis for tandem MS analysis but a significant limitation is that only charged fragments from ion dissociation events are detected while neutral fragments are simply lost. This study reports our continued effort to solve this problem by developing atmospheric pressure neutral reionization mass spectrometry (APNR). In APNR, analyte ions are thermally dissociated (at...

Photoswitchable interlocked thiodiglycolamide as a cocatalyst of a chalcogeno-Baylis–Hillman reaction† †Electronic supplementary information (ESI) available: Experimental procedures, spectroscopic and mass spectrometry data for all of the new compounds, and the full crystallographic details of 3a. CCDC 1532346. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c7sc00724h Click here for additional data file. Click here for additional data file.

Artificial disulfide-rich peptide scaffolds with precisely defined disulfide patterns and a minimized number of isomers† †Electronic supplementary information (ESI) available: Experiments on the synthesis and characterization of peptides, the oxidative folding of peptides, and the tryptic digestion LC-MS analysis of disulfide connectivity. See DOI: 10.1039/c6sc05710a Click here for additional data file.